[RASMB] how to determine base of cell

[Walter Stafford]

Hi Karl,
	Another way to do this would be to fill the cell with water 
and locate the bottoms from those traces. If you are using 
interference optics for equilibrium runs, you must take water optical 
blanks anyway. If you are using absorbance optics, you can set the 
wavelength so some non-absorbing region and take a scan. However, if 
there is a steep concentration gradient near the bottom that deflects 
the light out of the optics, that will not work. So you would have to 
do a water vs water run in that case too.

Walter Stafford

>Dear RASMB-ers,
>
>I am performing a series of sedimentation equilibrium experiments 
>using the 6 cell centerpieces.  For my analysis, I need to determine 
>accurately the radial position that corresponds to the bottom of 
>each chamber in the centerpiece.  It seems straightforward to 
>determine the miniscus of each chamber, but I am not sure of the 
>best way to go about determining the bottom of each chamber.  Any 
>help, and especially any literature references will be greatly 
>appreciated.
>
>N. Karl Maluf
>U. of Colorado Health Sciences Center
>Denver, CO.


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Walter F. Stafford III, Ph.D.
Senior Scientist and Director of the
Analytical Ultracentrifugation Research Laboratory
Boston Biomedical Research Institute
64 Grove Street
Watertown, MA  02472-2829

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