[RASMB] how to determine base of cell

[Karl.Maluf at UCHSC.edu]

Dear RASMB-ers,
 
I am performing a series of sedimentation equilibrium experiments using the 6 cell centerpieces.  For my analysis, I need to determine accurately the radial position that corresponds to the bottom of each chamber in the centerpiece.  It seems straightforward to determine the miniscus of each chamber, but I am not sure of the best way to go about determining the bottom of each chamber.  Any help, and especially any literature references will be greatly appreciated.
 
N. Karl Maluf
U. of Colorado Health Sciences Center
Denver, CO.
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