[RASMB] Chervenka centrepiece

[Jacob Lebowitz]

Yes the Chervenka (Griffiith see below) centerpiece was designed to achieve 
rapid equilibrium. I sent the following message yesterday but the CC to go 
to the entire RASMB list did not work.

Dear Tom, Iris and all RASMBers

Re Tom Laue's recent email, we did work on the "rapid equilibrium layering 
centerpiece" in 1970. Around 1996 I thought it might be worth pursuing a 
new layering centerpiece with more filling holes and I probably discussed 
this with Tom. I suspect that Tom recalls this as being not too long ago. 
My response re our work was to Iris only. However, Since there have been a 
number of responses re the 3 well layering centerpiece I provide the 
message to Iris below to those that may have an interest in the layering 
cell.  New fabrication of layering centerpieces and testing never took off 
since my other projects were of much higher priority.

Jack


Iris,

Many ages ago I was very impressed with using the Nazarian form of the sed. 
equil. equation and the layering rapid equilibrium centerpiece and decided 
to test both out with a number of proteins. Actually the Centerpiece was 
designed by Owen Griffith. The paper we published is DiCammelli, R.F., P.D. 
Holohan, S.F. Basinger and J. Lebowitz.  1970. Molecular weight 
determinations by low-speed sedimentation equilibrium.  A combined use of 
the Nazarian equation and the Griffith rapid equilibrium technique.  Anal. 
Biochem.,36:470-494. I believe that we had a figure in the paper of the 
centerpiece and of course references to the original publication, Chervenka 
or Griffith? I think our paper will help you out a lot.







At 05:44 PM 7/17/03 +0200, Richard M Thomas wrote:
>Wasn't the cell that Ariel describes designed to create a kind of 'instant 
>equilibrium' by layering decreasing concentrations of the sample on top of 
>one another (as it were) ?  Is this what is required here or would a 
>simpler synthetic boundary or band-forming centrepiece do the job?
>Regards to all
>Richard
>
>Dr Richard M Thomas
>Institut für Polymere
>ETH-Zentrum
>CH-8092 Zürich
>Switzerland
>+41 1 632 5540
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