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Yes the Chervenka (Griffiith see below) centerpiece was designed to
achieve rapid equilibrium. I sent the following message yesterday but the
CC to go to the entire RASMB list did not work.<br>
<br>
Dear Tom, Iris and all RASMBers<br>
<br>
Re Tom Laue's recent email, we did work on the "rapid equilibrium
layering centerpiece" in 1970. Around 1996 I thought it might be
worth pursuing a new layering centerpiece with more filling holes and I
probably discussed this with Tom. I suspect that Tom recalls this as
being not too long ago. My response re our work was to Iris only.
However, Since there have been a number of responses re the 3 well
layering centerpiece I provide the message to Iris below to those that
may have an interest in the layering cell. New fabrication of
layering centerpieces and testing never took off since my other projects
were of much higher priority. <br>
<br>
Jack <br>
<br>
<br>
Iris,<br>
<br>
Many ages ago I was very impressed with using the Nazarian form of the
sed. equil. equation and the layering rapid equilibrium centerpiece and
decided to test both out with a number of proteins. Actually the
Centerpiece was designed by Owen Griffith. The paper we published is
DiCammelli, R.F., P.D. Holohan, S.F. Basinger and J. Lebowitz.
1970. Molecular weight determinations by low-speed sedimentation
equilibrium. A combined use of the Nazarian equation and the
Griffith rapid equilibrium technique. Anal. Biochem.,36:470-494. I
believe that we had a figure in the paper of the centerpiece and of
course references to the original publication, Chervenka or Griffith? I
think our paper will help you out a lot. <br>
<br>
<br>
<br>
<br>
<br>
<br>
<br>
At 05:44 PM 7/17/03 +0200, Richard M Thomas wrote:<br>
<blockquote type=cite cite>Wasn't the cell that Ariel describes designed
to create a kind of 'instant equilibrium' by layering decreasing
concentrations of the sample on top of one another (as it were) ?
Is this what is required here or would a simpler synthetic boundary or
band-forming centrepiece do the job?<br>
Regards to all<br>
Richard<br>
<br>
<font face="Times New Roman, Times">Dr Richard M Thomas<br>
Institut für Polymere<br>
ETH-Zentrum<br>
CH-8092 Zürich<br>
Switzerland<br>
+41 1 632 5540</font> </blockquote></html>