[RASMB] hydrated vs. anhydrous MW

[Karl.Maluf at UCHSC.edu]

Dear RASMBers,
 
The typical hydration estimate for proteins of 0.3 grams water bound per gram protein would seem to predict that proteins will have molecular weights about 1.3 times larger than predicted by their primary sequences, yet this does not seem to be observed experimentally by sedimentation equilibrium techniques.  Can someone help me understand why this is not observed?
 
Karl Maluf