[RASMB] Lithium acetate

[Curth.Ute at mh-hannover.de]

Dear all,

a collaboration partner asked me whether we could  perform an SV run under conditions he used for protein crystallization (1.8 M Lithium acetate, 100 mM HEPES pH 7.4, very high protein concentration).
Does anyone have experience with interference detection at such high concentrations of LiAc? I guess at least exact chemical match of the reference buffer and meniscus matching are highly critical.
I did not find LiAc in the chemical resistances table of Beckman, is it expected to do any harm to epon centerpieces or aluminum housings?
Any comments are highly welcome,

Ute

--------------------------------
Prof. Dr. Ute Curth
Hannover Medical School
Research Core Unit for Structural Analysis, OE 8830
Carl-Neuberg-Str. 1
30625 Hannover
Germany

Phone:+49 511 5329372
Fax :+49 511 5325966
E-mail: Curth.Ute at mh-hannover.de

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