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<p class="MsoNormal"><span lang="DE">Dear all,<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="DE"><o:p> </o:p></span></p>
<p class="MsoNormal">a collaboration partner asked me whether we could perform an SV run under conditions he used for protein crystallization (1.8 M Lithium acetate, 100 mM HEPES pH 7.4, very high protein concentration).
<o:p></o:p></p>
<p class="MsoNormal">Does anyone have experience with interference detection at such high concentrations of LiAc? I guess at least exact chemical match of the reference buffer and meniscus matching are highly critical.
<o:p></o:p></p>
<p class="MsoNormal">I did not find LiAc in the chemical resistances table of Beckman, is it expected to do any harm to epon centerpieces or aluminum housings?<o:p></o:p></p>
<p class="MsoNormal">Any comments are highly welcome,<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Ute<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><span style="font-family:"Times New Roman","serif"">--------------------------------<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Times New Roman","serif"">Prof. Dr. Ute Curth</span><span style="font-family:"Times New Roman","serif";color:black"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Times New Roman","serif";color:black">Hannover Medical School
<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Times New Roman","serif";color:black">Research Core Unit for Structural Analysis, OE 8830
<br>
Carl-Neuberg-Str. 1 <br>
30625 Hannover <br>
Germany <o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Times New Roman","serif""><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Times New Roman","serif";color:black">Phone:+49 511 5329372
<br>
Fax :+49 511 5325966 <o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Times New Roman","serif";color:black">E-mail: Curth.Ute@mh-hannover.de<o:p></o:p></span></p>
<p class="MsoNormal"><o:p> </o:p></p>
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