[RASMB] dn/dc

[Stephen McLaughlin]

Dear Mattia, 

at the minute, it would be good to know if the dn/dc increases or decreases  and roughy how much. There is a significant discrepancy between the molar mass measured by SEC-MALS and the expected mass from mass-spec. 

Best 

Stephen



On 17 Mar 2016, at 09:53, Mattia Rocco wrote:

> Dear Stephen,
> 
> it likely will. But calculating a dn/dc is not that reliable anyway... and what's the level of precision you need, after all?
> 
> Best - Mattia
> 
> ________________________________
> 
> From: RASMB on behalf of Stephen McLaughlin
> Sent: Thu 3/17/2016 10:40 AM
> To: rasmb at rasmb.org
> Subject: [RASMB] dn/dc
> 
> 
> Dear all,  
> 
> I have a small three fingered Zn binding protein.  Will the presence of Zn ions affect the refractive increment that can be caluculated from sequence?
> 
> 
> Best 
> 
> Stephen
> 
> 
> ______________________________
> 
> Stephen McLaughlin, Ph.D.
> 
> Biophysics
> MRC Laboratory of Molecular Biology
> Francis Crick Avenue
> Cambridge Biomedical Campus
> Cambridge, CB2 0QH
> 
> Tel.: +44 (0) 1223 267891 (office)
> 
> Email:stephenm at mrc-lmb.cam.ac.uk
> _____________________________
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> 
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> 
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>