[RASMB] concentration range for non-ideal behavior

[John Sumida]

Regarding my email below, I'd just like to thank all for taking the time to
provide their any comments and insights in response to my question.

 

Best regards

John Sumida

University of Washington

 

From: John Sumida [mailto:jpsumida at u.washington.edu] 
Sent: Tuesday, January 20, 2015 6:01 PM
To: rasmb at rasmb.org
Subject: concentration range for non-ideal behavior

 

Dear RASMB,

 

My recollection is that concentrations in excess of 1.0 mg/ml should be
avoided due to the possibility of non-ideal interactions that would
complicate the analysis of SV and SE measurements.  However in two recent
cases (in one case a mAb was being studied, and in another a globular viral
capsid protein), we exceeded this value by a factor of five and failed to
observe any evidence of non-ideal behavior. In both cases, it was possible
to fit both SV and SE data without assuming any non-ideal interactions when
the experiment was performed in PBS or HEPES.

 

I would be curious to hear if it is the experience of the community that
non-ideality is commonly to be expected at concentrations >1.0mg/ml or
whether the concentration cutoff for non-ideality is less of an actual
cutoff being more a function of the particular properties of the molecule
being studied.

 

Best regards

John Sumida

University of Washington

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