[RASMB] concentration range for non-ideal behavior

[John Sumida]

Dear RASMB,

 

My recollection is that concentrations in excess of 1.0 mg/ml should be
avoided due to the possibility of non-ideal interactions that would
complicate the analysis of SV and SE measurements.  However in two recent
cases (in one case a mAb was being studied, and in another a globular viral
capsid protein), we exceeded this value by a factor of five and failed to
observe any evidence of non-ideal behavior. In both cases, it was possible
to fit both SV and SE data without assuming any non-ideal interactions when
the experiment was performed in PBS or HEPES.

 

I would be curious to hear if it is the experience of the community that
non-ideality is commonly to be expected at concentrations >1.0mg/ml or
whether the concentration cutoff for non-ideality is less of an actual
cutoff being more a function of the particular properties of the molecule
being studied.

 

Best regards

John Sumida

University of Washington

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