[RASMB] Volatile buffers in AUC question

[David Hayes]

Hi John,
I was told that the outlets of the vacuum pumps went right into the inside of the centrifuge:  so the leak from the cell would be expelled as a vapor into the chassis of the centrifuge.Similarly, if a botox sample cell leaked, the toxin could go just about anywhere and they would have to throw away the centrifuge:  (a second hand apocryphal story from Allergan).
Kind Regards,
David

 

     From: John Philo <jphilo at mailway.com>
 To: 'David Hayes' <drdavidbhayes at yahoo.com>; 'RASMB' <rasmb at rasmb.org> 
 Sent: Wednesday, October 14, 2015 1:21 PM
 Subject: RE: [RASMB] Volatile buffers in AUC question
   
#yiv6391599244 -- filtered {font-family:Helvetica;panose-1:2 11 6 4 2 2 2 2 2 4;}#yiv6391599244 filtered {panose-1:2 4 5 3 5 4 6 3 2 4;}#yiv6391599244 filtered {font-family:Calibri;panose-1:2 15 5 2 2 2 4 3 2 4;}#yiv6391599244 p.yiv6391599244MsoNormal, #yiv6391599244 li.yiv6391599244MsoNormal, #yiv6391599244 div.yiv6391599244MsoNormal {margin:0in;margin-bottom:.0001pt;font-size:12.0pt;}#yiv6391599244 a:link, #yiv6391599244 span.yiv6391599244MsoHyperlink {color:#0563C1;text-decoration:underline;}#yiv6391599244 a:visited, #yiv6391599244 span.yiv6391599244MsoHyperlinkFollowed {color:#954F72;text-decoration:underline;}#yiv6391599244 span.yiv6391599244EmailStyle17 {color:blue;font-weight:normal;font-style:normal;text-decoration:none none;}#yiv6391599244 .yiv6391599244MsoChpDefault {font-size:10.0pt;}#yiv6391599244 filtered {margin:1.0in 1.0in 1.0in 1.0in;}#yiv6391599244 div.yiv6391599244WordSection1 {}#yiv6391599244 David, if it were leaking into vacuum (no oxygen), how could it burn? Am I missing something?  John  

From: RASMB [mailto:rasmb-bounces at list.rasmb.org] On Behalf Of David Hayes
Sent: Wednesday, October 14, 2015 9:59 AM
To: RASMB <rasmb at rasmb.org>
Subject: [RASMB] Volatile buffers in AUC question  Hi all, I have a sample where we want to run in a buffer with 10% IPA.In general, because of the possibility of ignition of flammable components if there was a cell leak, People at AUC workshops say not to run organic solvents in the Beckman XLA/XLI instruments.Should I be worried about this buffer with 10% isopropyl alcohol?  Even if a cell leaked and 40 micro liters of IPA evaporated along with the aqueous components, how big of a flash would it be?  Could it damage the instrument? I know that polymer chemists also run AUC and wonder if there is any actual experience with organic solvents and any verified incidents of cells leaking and causing fire damage. Kind Regards, David Hayes

  
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