<html><head></head><body><div style="color:#000; background-color:#fff; font-family:HelveticaNeue, Helvetica Neue, Helvetica, Arial, Lucida Grande, sans-serif;font-size:16px"><div id="yiv6391599244"><div id="yui_3_16_0_1_1444844725642_3366"><div style="color:#000;background-color:#fff;font-family:HelveticaNeue, Helvetica Neue, Helvetica, Arial, Lucida Grande, sans-serif;font-size:16px;" id="yui_3_16_0_1_1444844725642_3365"><div id="yiv6391599244yui_3_16_0_1_1444844383306_4805"><span>Hi John,</span></div><div id="yiv6391599244yui_3_16_0_1_1444844383306_4806"><span><br clear="none"></span></div><div id="yiv6391599244yui_3_16_0_1_1444844383306_4808"><span id="yiv6391599244yui_3_16_0_1_1444844383306_4807">I was told that the outlets of the vacuum pumps went right into the inside of the centrifuge: so the leak from the cell would be expelled as a vapor into the chassis of the centrifuge.</span></div><div id="yiv6391599244yui_3_16_0_1_1444844383306_4810"><span id="yiv6391599244yui_3_16_0_1_1444844383306_4809">Similarly, if a botox sample cell leaked, the toxin could go just about anywhere and they would have to throw away the centrifuge: (a second hand apocryphal story from Allergan).</span></div><div id="yiv6391599244yui_3_16_0_1_1444844383306_4810"><span><br clear="none"></span></div><div id="yiv6391599244yui_3_16_0_1_1444844383306_4810">Kind Regards,</div><div id="yiv6391599244yui_3_16_0_1_1444844383306_4810"><br clear="none"></div><div id="yiv6391599244yui_3_16_0_1_1444844383306_4810">David</div><div id="yiv6391599244yui_3_16_0_1_1444844383306_4810"><br clear="none"></div><br clear="none"> <div class="qtdSeparateBR"><br><br></div><div class="yiv6391599244yqt7182164777" id="yiv6391599244yqt89152"><div id="yiv6391599244yui_3_16_0_1_1444844383306_4699" style="font-family:HelveticaNeue, Helvetica Neue, Helvetica, Arial, Lucida Grande, sans-serif;font-size:16px;"> <div id="yiv6391599244yui_3_16_0_1_1444844383306_4698" style="font-family:HelveticaNeue, Helvetica Neue, Helvetica, Arial, Lucida Grande, sans-serif;font-size:16px;"> <div dir="ltr" id="yiv6391599244yui_3_16_0_1_1444844383306_4697"> <hr id="yiv6391599244yui_3_16_0_1_1444844383306_4811" size="1"> <font id="yiv6391599244yui_3_16_0_1_1444844383306_4696" size="2" face="Arial"> <b><span style="font-weight:bold;">From:</span></b> John Philo <jphilo@mailway.com><br clear="none"> <b><span style="font-weight:bold;">To:</span></b> 'David Hayes' <drdavidbhayes@yahoo.com>; 'RASMB' <rasmb@rasmb.org> <br clear="none"> <b><span style="font-weight:bold;">Sent:</span></b> Wednesday, October 14, 2015 1:21 PM<br clear="none"> <b><span style="font-weight:bold;">Subject:</span></b> RE: [RASMB] Volatile buffers in AUC question<br clear="none"> </font> </div> <div class="yiv6391599244y_msg_container" id="yiv6391599244yui_3_16_0_1_1444844383306_4704"><br clear="none"><div id="yiv6391599244"><style>#yiv6391599244 --
filtered {font-family:Helvetica;panose-1:2 11 6 4 2 2 2 2 2 4;}
#yiv6391599244 filtered {panose-1:2 4 5 3 5 4 6 3 2 4;}
#yiv6391599244 filtered {font-family:Calibri;panose-1:2 15 5 2 2 2 4 3 2 4;}
#yiv6391599244
p.yiv6391599244MsoNormal, #yiv6391599244 li.yiv6391599244MsoNormal, #yiv6391599244 div.yiv6391599244MsoNormal
{margin:0in;margin-bottom:.0001pt;font-size:12.0pt;}
#yiv6391599244 a:link, #yiv6391599244 span.yiv6391599244MsoHyperlink
{color:#0563C1;text-decoration:underline;}
#yiv6391599244 a:visited, #yiv6391599244 span.yiv6391599244MsoHyperlinkFollowed
{color:#954F72;text-decoration:underline;}
#yiv6391599244 span.yiv6391599244EmailStyle17
{color:blue;font-weight:normal;font-style:normal;text-decoration:none none;}
#yiv6391599244 .yiv6391599244MsoChpDefault
{font-size:10.0pt;}
#yiv6391599244 filtered {margin:1.0in 1.0in 1.0in 1.0in;}
#yiv6391599244 div.yiv6391599244WordSection1
{}
#yiv6391599244 </style><div id="yiv6391599244yui_3_16_0_1_1444844383306_4703"><div class="yiv6391599244WordSection1" id="yiv6391599244yui_3_16_0_1_1444844383306_4702"><div class="yiv6391599244MsoNormal" id="yiv6391599244yui_3_16_0_1_1444844383306_4701"><span id="yiv6391599244yui_3_16_0_1_1444844383306_4700" style="font-size:10.0pt;">David, if it were leaking into vacuum (no oxygen), how could it burn? Am I missing something?</span></div><div class="yiv6391599244MsoNormal" id="yiv6391599244yui_3_16_0_1_1444844383306_4705"><span style="font-size:10.0pt;"> </span></div><div class="yiv6391599244MsoNormal" id="yiv6391599244yui_3_16_0_1_1444844383306_4706"><span style="font-size:10.0pt;">John</span></div><div class="yiv6391599244MsoNormal" id="yiv6391599244yui_3_16_0_1_1444844383306_4707"><span style="font-size:10.0pt;"> </span></div><div class="yiv6391599244qtdSeparateBR" id="yui_3_16_0_1_1444844725642_3420"><br clear="none"><br clear="none"></div><div class="yiv6391599244yqt9603541988" id="yiv6391599244yqt97857"><div id="yiv6391599244yui_3_16_0_1_1444844383306_4709"><div id="yiv6391599244yui_3_16_0_1_1444844383306_4708" style="border:none;border-top:solid #E1E1E1 1.0pt;padding:3.0pt 0in 0in 0in;"><div class="yiv6391599244MsoNormal" id="yiv6391599244yui_3_16_0_1_1444844383306_4710"><b><span style="font-size:11.0pt;">From:</span></b><span id="yiv6391599244yui_3_16_0_1_1444844383306_4711" style="font-size:11.0pt;"> RASMB [mailto:rasmb-bounces@list.rasmb.org] <b>On Behalf Of </b>David Hayes<br clear="none"><b>Sent:</b> Wednesday, October 14, 2015 9:59 AM<br clear="none"><b>To:</b> RASMB <rasmb@rasmb.org><br clear="none"><b>Subject:</b> [RASMB] Volatile buffers in AUC question</span></div></div></div><div class="yiv6391599244MsoNormal" id="yiv6391599244yui_3_16_0_1_1444844383306_4712"> </div><div id="yiv6391599244yui_3_16_0_1_1444844383306_4714"><div id="yiv6391599244yui_3_16_0_1_1444841906429_2631"><div class="yiv6391599244MsoNormal" id="yiv6391599244yui_3_16_0_1_1444844383306_4713" style="background:white;"><span style="">Hi all,</span></div></div><div id="yiv6391599244yui_3_16_0_1_1444841906429_2635"><div class="yiv6391599244MsoNormal" id="yiv6391599244yui_3_16_0_1_1444844383306_4715" style="background:white;"><span style=""> </span></div></div><div id="yiv6391599244yui_3_16_0_1_1444841906429_2639"><div class="yiv6391599244MsoNormal" id="yiv6391599244yui_3_16_0_1_1444844383306_4716" style="background:white;"><span style="" id="yui_3_16_0_1_1444844725642_3421">I have a sample where we want to run in a buffer with 10% IPA.</span></div></div><div id="yiv6391599244yui_3_16_0_1_1444841906429_2643"><div class="yiv6391599244MsoNormal" id="yiv6391599244yui_3_16_0_1_1444844383306_4718" style="background:white;"><span id="yiv6391599244yui_3_16_0_1_1444844383306_4717" style="">In general, because of the possibility of ignition of flammable components if there was a cell leak, People at AUC workshops say not to run organic solvents in the Beckman XLA/XLI instruments.</span></div></div><div id="yiv6391599244yui_3_16_0_1_1444841906429_2647"><div class="yiv6391599244MsoNormal" style="background:white;" id="yui_3_16_0_1_1444844725642_3423"><span style="" id="yui_3_16_0_1_1444844725642_3422">Should I be worried about this buffer with 10% isopropyl alcohol? </span></div></div><div id="yiv6391599244yui_3_16_0_1_1444841906429_2651"><div class="yiv6391599244MsoNormal" style="background:white;" id="yui_3_16_0_1_1444844725642_3425"><span style="" id="yui_3_16_0_1_1444844725642_3424">Even if a cell leaked and 40 micro liters of IPA evaporated along with the aqueous components, how big of a flash would it be? Could it damage the instrument?</span></div></div><div id="yiv6391599244yui_3_16_0_1_1444841906429_2655"><div class="yiv6391599244MsoNormal" style="background:white;"><span style=""> </span></div></div><div id="yiv6391599244yui_3_16_0_1_1444841906429_2659"><div class="yiv6391599244MsoNormal" style="background:white;"><span style="">I know that polymer chemists also run AUC and wonder if there is any actual experience with organic solvents and any verified incidents of cells leaking and causing fire damage.</span></div></div><div id="yiv6391599244yui_3_16_0_1_1444841906429_2663"><div class="yiv6391599244MsoNormal" style="background:white;"><span style=""> </span></div></div><div id="yiv6391599244yui_3_16_0_1_1444841906429_2667"><div class="yiv6391599244MsoNormal" style="background:white;"><span style="">Kind Regards,</span></div></div><div id="yiv6391599244yui_3_16_0_1_1444841906429_2671"><div class="yiv6391599244MsoNormal" style="background:white;"><span style=""> </span></div></div><div id="yiv6391599244yui_3_16_0_1_1444841906429_2675"><div class="yiv6391599244MsoNormal" style="background:white;"><span style="">David Hayes</span></div></div></div></div></div></div></div><br clear="none"><br clear="none"></div> </div> </div></div> </div></div></div></div></body></html>