[RASMB] density measurements

[John Sumida]

Dear Ariel,

Thank you for the reference.  Also thanks to all the very helpful comments
provided in response to my post.  RASMB has been and continues to be a
valuable resource for me and our small group here.

Best regards
John Sumida

-----Original Message-----
From: RASMB [mailto:rasmb-bounces at list.rasmb.org] On Behalf Of Ariel Lustig
Sent: Wednesday, October 01, 2014 2:48 AM
To: rasmb
Subject: [RASMB] density mesuraments

Dear John  Sumida, dear  colleagues,
Since  i`m long  time  retired, I can give only  a small  hint of my  old
experience of  measuring densities. it  is  a  fact that every  methods has
their  
own  pitfalls !!! and  advances !!!  p.e. using  density with  the
Analytical ultracentrifuge you  avoid  air  bubbles  in the  solution
.I
like to give  a  reference of  a method we  developed appeared  in
Biochimica et  Biophysica  Acta 1464 (2000) 199-206. <<Molecular weight
determination of  membrane proteins by  sedimentation equilibrium at sucrose
or  Nycodenz -adjusted density of the  hydrated  detergent  micelle<< by
Ariel  Lustig,
Andreas Engel, Georgias Tsiotis, Ehud M Landau, Werner Baschong . At
reference N° 7 of  the mentioned paper , is  described a different  methode
to measure density  of  particles with  the   AUC.
With best  regards
.ariel
ariel.lustig at bluewin.ch

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