[RASMB] density mesuraments

Ariel Lustig ariel.lustig at bluewin.ch
Wed Oct 1 02:47:49 PDT 2014


Dear John  Sumida, dear  colleagues,
Since  i`m long  time  retired, I can give only  a small  hint of my  old experience of  measuring densities. it  is  a  fact that every  methods has  their  
own  pitfalls !!! and  advances !!!  p.e. using  density with  the   Analytical ultracentrifuge you  avoid  air  bubbles  in the  solution….I like to give  a  reference of  a method we  developed appeared  in  Biochimica et  Biophysica  Acta 1464 (2000) 199-206. <<Molecular weight determination of  membrane proteins by  sedimentation equilibrium at sucrose or  Nycodenz -adjusted density of the  hydrated  detergent  micelle<< by  Ariel  Lustig,
Andreas Engel, Georgias Tsiotis, Ehud M Landau, Werner Baschong . At  reference N° 7 of  the mentioned paper , is  described a different  methode
to measure density  of  particles with  the   AUC.
With best  regards….ariel
ariel.lustig at bluewin.ch



More information about the RASMB mailing list