[RASMB] density mesuraments
Ariel Lustig
ariel.lustig at bluewin.ch
Wed Oct 1 02:47:49 PDT 2014
Dear John Sumida, dear colleagues,
Since i`m long time retired, I can give only a small hint of my old experience of measuring densities. it is a fact that every methods has their
own pitfalls !!! and advances !!! p.e. using density with the Analytical ultracentrifuge you avoid air bubbles in the solution….I like to give a reference of a method we developed appeared in Biochimica et Biophysica Acta 1464 (2000) 199-206. <<Molecular weight determination of membrane proteins by sedimentation equilibrium at sucrose or Nycodenz -adjusted density of the hydrated detergent micelle<< by Ariel Lustig,
Andreas Engel, Georgias Tsiotis, Ehud M Landau, Werner Baschong . At reference N° 7 of the mentioned paper , is described a different methode
to measure density of particles with the AUC.
With best regards….ariel
ariel.lustig at bluewin.ch
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