[RASMB] SPR query about CFCA applications

[Mattia Rocco]

The f/f0 Natalia mentions is for almost spherical shape.... if you are 
dealing with an antibody, then the 1.5 value might be more appropriate (but 
I haven't done any calculations on it... ;-) )

Best - Mattia

At 08:13 AM 1/14/2014 +0000, Markova, Natalia (GE Healthcare) wrote:
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>Hi John,
>
>
>
>We use as default f/f0 factor for globular proteins, which is 1.2 (not 
>1.5). However, user can freely enter any value for this factor in Web 
>diffusion coefficient calculator 
>(<https://www.biacore.com/lifesciences/Application_Support/online_support/Diffusion_Coefficient_Calculator/index.html?section=lifesciences&realsection=lifesciences>https://www.biacore.com/lifesciences/Application_Support/online_support/Diffusion_Coefficient_Calculator/index.html?section=lifesciences&realsection=lifesciences)
>
>You need to be registered on the Biacore website to access it.
>
>
>
>Best regards,
>
>
>
>Natalia
>
>
>
>Natalia Markova (PhD)
>
>GE Healthcare
>
>R&D Life Sciences
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>From: rasmb-bounces at list.rasmb.org [mailto:rasmb-bounces at list.rasmb.org] 
>On Behalf Of John Sumida
>Sent: den 14 januari 2014 00:20
>To: rasmb at rasmb.org
>Subject: [RASMB] SPR query about CFCA applications
>
>
>
>Dear RASMB,
>
>
>
>This question is in regards to an SPR application.  We are currently 
>investigating the use calibration free concentration analysis (CFCA) to 
>monitor the stability of macromolecular preps over time in terms of their 
>activity.  Input parameters for this analysis are ff0, viscosity, MW and D 
>and we are using AUC to better determine both.  Current applications seem 
>to assume ff0=1.5, which may not be correct for monoclonal preps based on 
>my reading of the literature.
>
>
>
>My query.
>
>1.       Is it currently the common practice to measure these quantities 
>and if so what is the preferred method to do so?
>
>
>
>Thank you all and Happy New Year,
>
>
>
>John Sumida, Ph.D.
>
><http://depts.washington.edu/cidb4bio/index.shtml>Analytical Biopharmacy 
>Core Facility
>
>University of Washington
>
><http://www.moles.washington.edu/>Molecular Engineering & Sciences 
>Institute, G22
>
>3946 West Stevens Way NE
>
>Seattle WA 98195-1653
>
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