<html>
<br>
The f/f0 Natalia mentions is for almost spherical shape.... if you are
dealing with an antibody, then the 1.5 value might be more appropriate
(but I haven't done any calculations on it... ;-) )<br><br>
Best - Mattia<br><br>
At 08:13 AM 1/14/2014 +0000, Markova, Natalia (GE Healthcare)
wrote:<br>
<blockquote type=cite class=cite cite>Content-Language: en-US<br>
Content-Type: multipart/alternative;<br>
<x-tab> </x-tab>boundary="_000_41C5780FD432C74DB68C588BE66E34F614EF6B76BUDURBPA08e2kad_"<br><br>
Hi John,<br><br>
<br><br>
We use as default f/f0 factor for globular proteins, which is 1.2 (not
1.5). However, user can freely enter any value for this factor in Web
diffusion coefficient calculator
(<a href="https://www.biacore.com/lifesciences/Application_Support/online_support/Diffusion_Coefficient_Calculator/index.html?section=lifesciences&realsection=lifesciences">https://www.biacore.com/lifesciences/Application_Support/online_support/Diffusion_Coefficient_Calculator/index.html?section=lifesciences&realsection=lifesciences</a>)<br><br>
You need to be registered on the Biacore website to access it.<br><br>
<br><br>
Best regards,<br><br>
<br><br>
Natalia<br><br>
<br><br>
<b><i>Natalia Markova (PhD) <br>
</i></b><br>
<i>GE Healthcare<br>
</i><br>
<i>R&D Life Sciences<br>
</i><br>
<i>Rapsgatan 23<br>
</i><br>
<i>751 84 Uppsala<br>
</i><br>
<i>Sweden<br>
</i><br>
<i> <br>
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M +46 727 33 58 80<br><br>
E
<a href="mailto:natalia.markova@ge.com">natalia.markova@ge.com</a><br><br>
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<b>From:</b> rasmb-bounces@list.rasmb.org
[<a href="mailto:rasmb-bounces@list.rasmb.org" eudora="autourl">mailto:rasmb-bounces@list.rasmb.org</a>]
<b>On Behalf Of </b>John Sumida<br>
<b>Sent:</b> den 14 januari 2014 00:20<br>
<b>To:</b> rasmb@rasmb.org<br>
<b>Subject:</b> [RASMB] SPR query about CFCA applications<br><br>
<br><br>
Dear RASMB,<br><br>
<br><br>
This question is in regards to an SPR application. We are currently
investigating the use calibration free concentration analysis (CFCA) to
monitor the stability of macromolecular preps over time in terms of their
activity. Input parameters for this analysis are ff0, viscosity, MW
and D and we are using AUC to better determine both. Current
applications seem to assume ff0=1.5, which may not be correct for
monoclonal preps based on my reading of the literature.<br><br>
<br><br>
My query.<br><br>
1. Is it currently the common
practice to measure these quantities and if so what is the preferred
method to do so?<br><br>
<br><br>
Thank you all and Happy New Year,<br><br>
<br><br>
John Sumida, Ph.D.<br><br>
<a href="http://depts.washington.edu/cidb4bio/index.shtml">Analytical
Biopharmacy Core Facility</a><br><br>
University of Washington<br><br>
<a href="http://www.moles.washington.edu/">Molecular Engineering &
Sciences Institute, G22</a><br><br>
3946 West Stevens Way NE<br><br>
Seattle WA 98195-1653<br><br>
<br>
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