[RASMB] varying temperature during an equilibrium run

[Walter Stafford]

I think if you want to be sure to be able to run at higher temperatures with interference optics, you should upgrade to a turbomolecular pump. It solved our problem completely. It leaves your chamber and lenses completely clean.

___________________________
Walter Stafford
wstafford3 at walterstafford.com

“Life is short, Break the Rules.
Forgive quickly, Kiss SLOWLY.
Love truly. Laugh uncontrollably
And never regret ANYTHING
That makes you smile.”

― Mark Twain


On Oct 25, 2013, at 4:30 PM, Kirk C Aune <kaune at sbcglobal.net> wrote:

> On 10/25/13 10:47, Frank Niesen wrote:
>> Hello,
>> 
>> I was wondering if anybody has experience with changing the temperature in an equilibrium run at given speed, to investigate temperature-dependent strength in association? Is it feasible/sane to record data at equilibrium at, e.g., 20C, and then to change the temperature to, e.g., 40C, and to hope to get data for equilibrium and record once more?
>> 
>> In my case, the association seems to weaken substantially with temperature and I observe a weaker curvature at the higher temperature. For a third step, going back to the original temperature, I programmed the run, but made two mistakes with it - firstly to not allow sufficient time for the equilibrium and, secondly, to somehow got the "stop AUC after last scan" checked :o(
>> As result, I am looking at a scan that doesn't resemble the one at lower temperature, yet it is similar to scans I saw during the equilibration between the two temperatures at step 2.
>> 
>> Has anybody tried something similar? Am I onto something believable/real, or shouldn't waste any more precious sample on a proper repeat (i.e. separate runs/samples for each temperature)?
>> 
>> Thanks in advance!
>> Frank
>> 
>> 
>> _______________________________________________
>> RASMB mailing list
>> RASMB at list.rasmb.org
>> http://list.rasmb.org/listinfo.cgi/rasmb-rasmb.org
> I refer you to Dimerization of Alpha-Chymotrypsin. II.  Ionic Strength and Temperature Dependence.  Biochemistry 10, no.9, pp 1617-1622 (1971).  Kirk C Aune, Lowell C. Goldsmith and Serge N. Timasheff.
> 
> This, of course, was with a Model E  AUC.  In order to get stable temperature control above 30C with no oil condensation, we employed an aluminum flashing liner on the chamber wall.
> 
> Cheers.
> 
> -- 
> Kirk C Aune, Ph.D.
> 16289 Cambridge Court
> Granger, IN 46530
> (574)277-8589
> Linux User #1720
> _______________________________________________
> RASMB mailing list
> RASMB at list.rasmb.org
> http://list.rasmb.org/listinfo.cgi/rasmb-rasmb.org

-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://list.rasmb.org/pipermail/rasmb-rasmb.org/attachments/20131025/902c9228/attachment-0001.html>