[RASMB] frictional ratio and asymmetry

[John Sumida]

Dear RASMB,

 

this question is in relation to f/f0. f/f0=(f/f)hydration*volume-asymetry
and is also equal to s-sphere/s20w as per Lebowitz et. al. (Protein Science
2002).  I have two questions.

 

1. what is the physical meaning of (f/f)hydration? Does this describe a
shape parameter of the un-hydrated macromolecule?

2. v-bar(solvent) is a factor in the volume-asymmetry term. How is this
related to the solvent density?

 

I also have a third related question.  Estimation of the axial ratio seems
to require some knowledge of s-0, the sedimentation value extrapolated to
zero macromolecule concentration.  Could someone please point me at an
appropriate reference describing how this is estimated in c(s).

 

Many thanks in advance, for this and all of my previous inquiries.

 

Best regards,

 

John Sumida, Ph.D.

 <http://depts.washington.edu/cidb4bio/index.shtml> Analytical Biopharmacy
Core Facility

University of Washington

 <http://www.moles.washington.edu/> Molecular Engineering & Sciences
Institute, G22

3946 West Stevens Way NE

Seattle WA 98195-1653

 

-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://list.rasmb.org/pipermail/rasmb-rasmb.org/attachments/20131119/1052772d/attachment.htm>