[RASMB] varying temperature during an equilibrium run

[Borries Demeler]

Frank,
in addition to the recommendations from the others I would like to mention that 
if you still use a diffusion pump to be careful with 40C, we have had excessive
oil deposits in the chamber when going to that high a temperature. Your machine
may work fine at this temp, but you better check before sticking the monochromator
into the chamber.

Regards, -Borries

On Fri, Oct 25, 2013 at 10:47:00AM -0400, Frank Niesen wrote:
> Hello,
> 
> I was wondering if anybody has experience with changing the temperature in
> an equilibrium run at given speed, to investigate temperature-dependent
> strength in association? Is it feasible/sane to record data at equilibrium
> at, e.g., 20C, and then to change the temperature to, e.g., 40C, and to
> hope to get data for equilibrium and record once more?
> 
> In my case, the association seems to weaken substantially with temperature
> and I observe a weaker curvature at the higher temperature. For a third
> step, going back to the original temperature, I programmed the run, but
> made two mistakes with it - firstly to not allow sufficient time for the
> equilibrium and, secondly, to somehow got the "stop AUC after last scan"
> checked :o(
> As result, I am looking at a scan that doesn't resemble the one at lower
> temperature, yet it is similar to scans I saw during the equilibration
> between the two temperatures at step 2.
> 
> Has anybody tried something similar? Am I onto something believable/real,
> or shouldn't waste any more precious sample on a proper repeat (i.e.
> separate runs/samples for each temperature)?
> 
> Thanks in advance!
> Frank

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