[RASMB] varying temperature during an equilibrium run

[Frank Niesen]

Hello,

I was wondering if anybody has experience with changing the temperature in
an equilibrium run at given speed, to investigate temperature-dependent
strength in association? Is it feasible/sane to record data at equilibrium
at, e.g., 20C, and then to change the temperature to, e.g., 40C, and to
hope to get data for equilibrium and record once more?

In my case, the association seems to weaken substantially with temperature
and I observe a weaker curvature at the higher temperature. For a third
step, going back to the original temperature, I programmed the run, but
made two mistakes with it - firstly to not allow sufficient time for the
equilibrium and, secondly, to somehow got the "stop AUC after last scan"
checked :o(
As result, I am looking at a scan that doesn't resemble the one at lower
temperature, yet it is similar to scans I saw during the equilibration
between the two temperatures at step 2.

Has anybody tried something similar? Am I onto something believable/real,
or shouldn't waste any more precious sample on a proper repeat (i.e.
separate runs/samples for each temperature)?

Thanks in advance!
Frank
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