[RASMB] Amyloid fibrils and Fujita approach

[Sophia Kenrick]

Sabine,

Jumping off of Ewa's comment, batch MALS is also useful for determining the size distribution of reversible amyloid oligomers.  I'm not sure if you're interested in the precursors to fibril formation or the final fibrils.  SEC-MALS is definitely suited for measuring the size distribution for larger, irreversible (or kinetically trapped) fibrils.  For small oligomers in equilibrium with monomers, you should observe a concentration-dependent Mw by batch MALS, similar to this study of insulin fibrils:  http://www.ncbi.nlm.nih.gov/pubmed/?term=10.1002%2Fbit.23188.

Sophia

-----Original Message-----
From: rasmb-bounces at list.rasmb.org [mailto:rasmb-bounces at list.rasmb.org] On Behalf Of Ewa Folta-Stogniew
Sent: Friday, April 05, 2013 10:36 AM
To: Sabine Kaltofen; rasmb at list.rasmb.org
Subject: Re: [RASMB] Amyloid fibrils and Fujita approach

Sabine,

I had analyzed abeta-peptide assembly distributions by SEC-MALLS.  Since MW determination from LS is independent of shape, SEC-MALLS is thus well suited for this heterogeneous system.

Ewa



At 02:34 PM 4/4/2013, Sabine Kaltofen wrote:
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>
>Dear all,
>
>has anyone of you tried to describe the size/molecular weight of 
>amyloid fibrils using the Fujita approach?
>I am after a rather accurate determination of the MW (distribution) of 
>fibrils.
>Therefore, using a scaling law to relate s with M seems the most 
>rigorous thing to do. I am, however not aware of any literature where 
>this approach has been used for peptide/protein fibrils.
>Am I missing a major point here, why this is not applicable?
>
>All comments, experiences and thoughts are greatly appreciated!
>
>Best wishes
>
>Sabine
>
>
>
>Mind your carbon footprint: please don't print this message.
>
>
>Sabine Kaltofen
>Biochemistry & Structural Biology
>Lund University
>sabine.kaltofen at biochemistry.lu.se
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>RASMB at list.rasmb.org
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