[RASMB] Is there a facile method to get Vbar for small molecules without using densitometry?

[Antje Voelkel]

Dear John,

there is the possibility to get the vbar value with equilibrium 
measurements in water and d2o:
*
The Simultaneous Determination of Partial Specific Volumes and Molecular 
Weights with Microgram Quantities
*/*S. J. EDELSTEIN AND H. K. SCHACHMAN
THE JOURNAL OF BIOLOGICAL CHEMISTRY Vol. 242, No. 2, Issue of January 
25. PP. 306-311, 1967*

/It is not really a "facile" method but it can help if one has just a 
little amount of sample.

Best regards
Antje



On 17.10.2012 23:21, john_doran at vrtx.com wrote:
> Greetings fellow Rasmb members
>
> The issue has arisen on more than one occasion for us to look at the 
> association state of small molecules ( i.e molecules under 1000 MW) 
> using the AUC. When there is plenty of material it is easy enough to 
> directly measure Vbar using densitometry, but of course there are also 
> occasions where the material is precious and there is not nearly 
> enough for densitometry. Does anyone know of any alternative physical 
> methods or getting Vbar values generated from theory that may be used 
> in lieu of densitometry?
>
> Thank you in advance for any replies that are sent to address this 
> problem!
>
>
> John D Doran, Ph.D.
> Protein Biochemistry
> Vertex Pharmaceuticals Inc.
> 130 Waverly Street
> Cambridge, MA 02139
> 617-444-6142
>
>
> _______________________________________________
> RASMB mailing list
> RASMB at rasmb.bbri.org
> http://rasmb.bbri.org/cgi-bin/mailman/listinfo/rasmb

-- 
Antje Voelkel
Max Planck Institute of Colloids and Interfaces
Am Muehlenberg 1
D-14476 Potsdam
+49(0)331 567 9510/9531

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