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Dear John,<br>
<br>
there is the possibility to get the vbar value with equilibrium
measurements in water and d2o:<br>
<b><br>
The Simultaneous Determination of Partial Specific Volumes and
Molecular Weights with Microgram Quantities<br>
</b><small><i><b>S. J. EDELSTEIN AND H. K. SCHACHMAN<br>
THE JOURNAL OF BIOLOGICAL CHEMISTRY Vol. 242, No. 2, Issue of
January 25. PP. 306-311, 1967</b><br>
<br>
</i></small>It is not really a "facile" method but it can help if
one has just a little amount of sample. <br>
<br>
Best regards<br>
Antje<br>
<br>
<br>
<br>
On 17.10.2012 23:21, <a class="moz-txt-link-abbreviated" href="mailto:john_doran@vrtx.com">john_doran@vrtx.com</a> wrote:
<blockquote
cite="mid:OF4EE31C81.534F1286-ON85257A9A.00741D46-85257A9A.007559DE@vrtx.com"
type="cite"><font face="sans-serif" size="2">Greetings fellow
Rasmb members</font><br>
<br>
<font face="sans-serif" size="2">The issue has arisen on more than
one
occasion for us to look at the association state of small
molecules ( i.e
molecules under 1000 MW) using the AUC. When there is plenty of
material
it is easy enough to directly measure Vbar using densitometry,
but of course
there are also occasions where the material is precious and
there is not
nearly enough for densitometry. Does anyone know of any
alternative physical
methods or getting Vbar values generated from theory that may be
used in
lieu of densitometry? </font><br>
<br>
<font face="sans-serif" size="2">Thank you in advance for any
replies
that are sent to address this problem!</font><br>
<br>
<br>
<font face="sans-serif" size="2">John D Doran, Ph.D.<br>
Protein Biochemistry<br>
Vertex Pharmaceuticals Inc.<br>
130 Waverly Street<br>
Cambridge, MA 02139<br>
617-444-6142</font><br>
<br>
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<br>
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</pre>
</blockquote>
<br>
<pre class="moz-signature" cols="72">--
Antje Voelkel
Max Planck Institute of Colloids and Interfaces
Am Muehlenberg 1
D-14476 Potsdam
+49(0)331 567 9510/9531</pre>
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