[RASMB] Is there a facile method to get Vbar for small molecules without using densitometry?

[john_doran at vrtx.com]

Greetings fellow Rasmb members

The issue has arisen on more than one occasion for us to look at the 
association state of small molecules ( i.e molecules under 1000 MW) using 
the AUC. When there is plenty of material it is easy enough to directly 
measure Vbar using densitometry, but of course there are also occasions 
where the material is precious and there is not nearly enough for 
densitometry. Does anyone know of any alternative physical methods or 
getting Vbar values generated from theory that may be used in lieu of 
densitometry? 

Thank you in advance for any replies that are sent to address this 
problem!


John D Doran, Ph.D.
Protein Biochemistry
Vertex Pharmaceuticals Inc.
130 Waverly Street
Cambridge, MA 02139
617-444-6142

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