[RASMB] Buffers for CD

[David Scott]

Hello All in RASMB-land,

I have a question on buffers for CD. I am performing CD experiments at different pH's. Obviously for pH 7, a nice optically clear phosphate buffer means I can see down to 185 nm on a Jasco 815 spectrometer. However, I am having problems getting buffers that are equally optically clear at pH 5.5 and pH 3.0. Any suggestions? Advice and comments on suitable sources of chemicals to make the buffer that don't have contaminating components in are also welcome.

Best wishes

Dave


>>>>>>>>>>>>>>>>>>>>>>>>>>
Dr. David J. Scott
Associate Professor and Reader in Physical Biochemistry
National Centre for Macromolecular Hydrodynamics
School of Biosciences
University of Nottingham
Sutton Bonington Campus
Leicestershire. LE12 5RD.
United Kingdom.
Phone: +44 (0) 115 951 6221
Fax: +44 (0) 115 951 6142
Email: dj.scott at nottingham.ac.uk
Web: www.nottingham.ac.uk/ncmh

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