<html xmlns:o="urn:schemas-microsoft-com:office:office" xmlns:w="urn:schemas-microsoft-com:office:word" xmlns:st1="urn:schemas-microsoft-com:office:smarttags" xmlns="http://www.w3.org/TR/REC-html40">
<head>
<META HTTP-EQUIV="Content-Type" CONTENT="text/html; charset=us-ascii">
<meta name=Generator content="Microsoft Word 11 (filtered medium)">
<o:SmartTagType namespaceuri="urn:schemas-microsoft-com:office:smarttags"
name="country-region"/>
<o:SmartTagType namespaceuri="urn:schemas-microsoft-com:office:smarttags"
name="PlaceType"/>
<o:SmartTagType namespaceuri="urn:schemas-microsoft-com:office:smarttags"
name="PlaceName"/>
<o:SmartTagType namespaceuri="urn:schemas-microsoft-com:office:smarttags"
name="place"/>
<!--[if !mso]>
<style>
st1\:*{behavior:url(#default#ieooui) }
</style>
<![endif]-->
<style>
<!--
/* Font Definitions */
@font-face
{font-family:SimSun;
panose-1:2 1 6 0 3 1 1 1 1 1;}
@font-face
{font-family:Georgia;
panose-1:2 4 5 2 5 4 5 2 3 3;}
@font-face
{font-family:"\@SimSun";
panose-1:2 1 6 0 3 1 1 1 1 1;}
/* Style Definitions */
p.MsoNormal, li.MsoNormal, div.MsoNormal
{margin:0cm;
margin-bottom:.0001pt;
font-size:12.0pt;
font-family:"Times New Roman";}
a:link, span.MsoHyperlink
{color:blue;
text-decoration:underline;}
a:visited, span.MsoHyperlinkFollowed
{color:purple;
text-decoration:underline;}
p
{mso-margin-top-alt:auto;
margin-right:0cm;
mso-margin-bottom-alt:auto;
margin-left:0cm;
font-size:12.0pt;
font-family:"Times New Roman";}
span.EmailStyle17
{mso-style-type:personal-compose;
font-family:Georgia;
color:windowtext;
font-weight:normal;
font-style:normal;
text-decoration:none none;}
@page Section1
{size:595.3pt 841.9pt;
margin:72.0pt 90.0pt 72.0pt 90.0pt;}
div.Section1
{page:Section1;}
-->
</style>
</head>
<body lang=EN-GB link=blue vlink=purple>
<div class=Section1>
<p class=MsoNormal><font size=2 face=Georgia><span style='font-size:10.0pt;
font-family:Georgia'>Hello All in RASMB-land,<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Georgia><span style='font-size:10.0pt;
font-family:Georgia'><o:p> </o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Georgia><span style='font-size:10.0pt;
font-family:Georgia'>I have a question on buffers for CD. I am performing CD
experiments at different pH’s. Obviously for pH 7, a nice optically clear
phosphate buffer means I can see down to 185 nm on a Jasco 815 spectrometer. However,
I am having problems getting buffers that are equally optically clear at pH 5.5
and pH 3.0. Any suggestions? Advice and comments on suitable sources of chemicals
to make the buffer that don’t have contaminating components in are also
welcome.<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Georgia><span style='font-size:10.0pt;
font-family:Georgia'><o:p> </o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Georgia><span style='font-size:10.0pt;
font-family:Georgia'>Best wishes<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Georgia><span style='font-size:10.0pt;
font-family:Georgia'><o:p> </o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Georgia><span style='font-size:10.0pt;
font-family:Georgia'>Dave<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Georgia><span style='font-size:10.0pt;
font-family:Georgia'><o:p> </o:p></span></font></p>
<p><font size=2 face=Georgia><span style='font-size:10.0pt;font-family:Georgia'>>>>>>>>>>>>>>>>>>>>>>>>>>></span></font>
<br>
<font size=2 face=Georgia><span style='font-size:10.0pt;font-family:Georgia'>Dr.
David J. Scott</span></font> <br>
<font size=2 face=Georgia><span style='font-size:10.0pt;font-family:Georgia'>Associate
Professor and Reader in Physical Biochemistry</span></font> <br>
<font size=2 face=Georgia><span style='font-size:10.0pt;font-family:Georgia'>National
Centre for Macromolecular Hydrodynamics</span></font> <br>
<font size=2 face=Georgia><span style='font-size:10.0pt;font-family:Georgia'>School
of <st1:PlaceName w:st="on">Biosciences</st1:PlaceName></span></font> <br>
<st1:PlaceType w:st="on"><font size=2 face=Georgia><span style='font-size:10.0pt;
font-family:Georgia'>University</span></font></st1:PlaceType><font size=2
face=Georgia><span style='font-size:10.0pt;font-family:Georgia'> of <st1:place
w:st="on">Nottingham</st1:place></span></font> <br>
<font size=2 face=Georgia><span style='font-size:10.0pt;font-family:Georgia'>Sutton
Bonington Campus</span></font> <br>
<font size=2 face=Georgia><span style='font-size:10.0pt;font-family:Georgia'>Leicestershire.
LE12 5RD.</span></font> <br>
<st1:country-region w:st="on"><st1:place w:st="on"><font size=2 face=Georgia><span
style='font-size:10.0pt;font-family:Georgia'>United Kingdom</span></font></st1:place></st1:country-region><font
size=2 face=Georgia><span style='font-size:10.0pt;font-family:Georgia'>.</span></font>
<br>
<font size=2 face=Georgia><span style='font-size:10.0pt;font-family:Georgia'>Phone:
+44 (0) 115 951 6221</span></font> <br>
<font size=2 face=Georgia><span style='font-size:10.0pt;font-family:Georgia'>Fax:
+44 (0) 115 951 6142</span></font> <br>
<font size=2 face=Georgia><span style='font-size:10.0pt;font-family:Georgia'>Email:
dj.scott@nottingham.ac.uk</span></font> <br>
<font size=2 face=Georgia><span style='font-size:10.0pt;font-family:Georgia'>Web:
www.nottingham.ac.uk/ncmh</span></font> <o:p></o:p></p>
<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'><o:p> </o:p></span></font></p>
</div>
<br/>
<p>
This message and any attachment are intended solely for the addressee and may
contain confidential information. If you have received this message in error,
please send it back to me, and immediately delete it. Please do not use,
copy or disclose the information contained in this message or in any attachment.
Any views or opinions expressed by the author of this email do not necessarily
reflect the views of the University of Nottingham.
</p>
<p>
This message has been checked for viruses but the contents of an attachment
may still contain software viruses which could damage your computer system:
you are advised to perform your own checks. Email communications with the
University of Nottingham may be monitored as permitted by UK legislation.
</p></body>
</html>