[RASMB] AUC in ficoll

[Titus M. Franzmann]

Thanks Jack,
Is there a good alternative that could be considered neutral in order to
test for a molecular crowding effect?
Best, 
Titus

-----Original Message-----
From: rasmb-bounces at rasmb.bbri.org [mailto:rasmb-bounces at rasmb.bbri.org] On
Behalf Of jack kornblatt
Sent: Wednesday, August 03, 2011 9:59 AM
To: rasmb at server1.bbri.org
Subject: [RASMB] AUC in ficoll

Hello All
It might be worth adding to the points mentioned that ficoll is a highly
branched polysaccharide and that it is not a "neutral" component of a
mixture.
There is an extensive literature on molecular crowding and osmotic stress.
Compounds such as dextran and PEG (and Ficoll by association) certainly
sediment but the osmotic pressure that they develop in the water column is
probably not by any stretch of the imagination non-negligible. Whether you
are in favour of the Timasheff explanation or the Parsegian explanation of
what the equations mean does not matter. 
What is important is recognizing that 100 mg/mL in your buffer is not a
simple analysis problem.
jack kornblatt
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