[RASMB] AUC of oligonucleotides

[Dmitry Veprintsev]

Dear Timothy,
from my experience with short oligos (12-24 bp),
Vbar of 0.5 seems to give expected molecular weight in equilibrium
run, at a reasonable ionic strength of 150-225 mM.
At lower ionic strength DNA seems to behave non-ideally - but you can
always run a control of DNA only to see what Vbar you should use.
regards, Dmitry


On Mon, Apr 4, 2011 at 2:10 PM, Timothy Dafforn <t.r.dafforn at bham.ac.uk> wrote:
> Hi all,
>
> I have a collaborator who would like to do some velocity runs of some short
> <8mer DNA oligomers.
>
> Has anyone done this sort of experiment?
>
> Does anyone have any ideas of the vbar values I should use for DNA?
>
> Cheers
>
> Tim
>
>
>
> School of Biosciences,
>
> University of Birmingham
>
> Edgbaston
>
> Birmingham
>
> West Midlands
>
> B15 2TT
>
> UK
>
>
>
> Tel +44 121 414 5881
>
>
>
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>



-- 
Dr. Dmitry Veprintsev
Biomolecular Research Laboratory, OFLC/103
Paul Scherrer Institut
5232 Villigen PSI
Switzerland
Tel +41 (0) 56 310 5246; Fax +41 (0)56 310 5288
dmitry.veprintsev at psi.ch
http://www.psi.ch/~veprintsev_d