[RASMB] Xl-I wavelength check question

[John Philo]

Mark, there is no need to use a cell at all. Simply place one standard cell
opposite the counterbalance, leaving the other holes empty, and then do the
wavelength check on one of the empty holes. Scanning an empty hole is also
the best way to do a noise check on the absorbance system.
 
John

  _____  

From: rasmb-bounces at rasmb.bbri.org [mailto:rasmb-bounces at rasmb.bbri.org] On
Behalf Of Mark Agacan
Sent: Wednesday, February 17, 2010 2:49 AM
To: rasmb at server1.bbri.org
Subject: [RASMB] Xl-I wavelength check question


I have a question regarding the wavelength accuracy check for the XL-I:
 
Using a windowless cell (aluminium cell housing only with no centerpiece or
windows, or one of the empty holes in the rotor?) - how can I match the
weight of this cell with the counterbalance, even at the relatively low
speed of 3000 rpm?

The remainder of the procedure as outlined in the Troubleshooting and
Maintenance section of the XL-I manual seems straighforward enough, i'm just
a little unclear on exactly how to load the rotor for this measurement.
 
Best Regards
 
Mark
 

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Dr Mark Agacan, Scientific Officer for the Division of Biological Chemistry
and Drug Discovery,
Wellcome Trust Biocentre, College of Life Sciences, University of Dundee,
Dundee, DD1 5EH 
Tel: +44 1382 386095 Fax: +44 1382 345764 Mobile: 07525 451 117
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~



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