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<DIV dir=ltr align=left><SPAN class=557221916-17022010><FONT size=2>Mark, there
is no need to use a cell at all. Simply place one standard cell opposite the
counterbalance, leaving the other holes empty, and then do the wavelength
check on one of the empty holes. Scanning an empty hole is also the
best way to do a noise check on the absorbance system.</FONT></SPAN></DIV>
<DIV dir=ltr align=left><SPAN class=557221916-17022010><FONT
size=2></FONT></SPAN> </DIV>
<DIV dir=ltr align=left><SPAN class=557221916-17022010><FONT
size=2>John</FONT></SPAN></DIV><BR>
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<FONT size=2><B>From:</B> rasmb-bounces@rasmb.bbri.org
[mailto:rasmb-bounces@rasmb.bbri.org] <B>On Behalf Of </B>Mark
Agacan<BR><B>Sent:</B> Wednesday, February 17, 2010 2:49 AM<BR><B>To:</B>
rasmb@server1.bbri.org<BR><B>Subject:</B> [RASMB] Xl-I wavelength check
question<BR></FONT><BR></DIV>
<DIV></DIV>
<DIV>I have a question regarding the wavelength accuracy check for the
XL-I:</DIV>
<DIV> </DIV>
<DIV>Using a windowless cell (aluminium cell housing only with no centerpiece or
windows, or one of the empty holes in the rotor?) - how
can I match the weight of this cell with the counterbalance, even at
the relatively low speed of 3000 rpm?<BR></DIV>
<DIV>The remainder of the procedure as outlined in the Troubleshooting and
Maintenance section of the XL-I manual seems straighforward enough, i'm just a
little unclear on exactly how to load the rotor for this measurement.</DIV>
<DIV> </DIV>
<DIV>Best Regards</DIV>
<DIV> </DIV>
<DIV>Mark</DIV>
<DIV> </DIV><BR>
<DIV
style="CLEAR: both">~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~<BR>Dr
Mark Agacan, Scientific Officer for the Division of Biological Chemistry and
Drug Discovery,<BR>Wellcome Trust Biocentre, College of Life Sciences,
University of Dundee, Dundee, DD1 5EH <BR>Tel: +44 1382 386095 Fax: +44 1382
345764 Mobile: 07525 451
117<BR>~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~</DIV><BR><BR><BR>
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