[RASMB] radial scans 6 sector cells

[John Philo]

Lake, it seems to me for equilibrium there is no need to capture any data to
the left of the meniscus, or even the meniscus itself. You'll just have to
trim all that region away before you analyze anyway. So the default start at
5.8 cm should capture everything you will need. On the other end I find
there is no useful (fittable) data beyond ~7.12 cm. Also remember that UV
does damage proteins, and that all three samples (3 channels) get exposed
with each flash, so at some point extra flashes to collect data you aren't
going to use does become harmful.
 
Roy, you may find it useful to have both a velocity method and an
equilibrium one open. I usually do a quick velocity-mode scan to be sure
everything is loaded okay and to figure out the radial range I want, and
then set up the equilibrium method window based on the quick velocity-mode
scan results.
 
John
 

  _____  

From: rasmb-bounces at rasmb.bbri.org [mailto:rasmb-bounces at rasmb.bbri.org] On
Behalf Of Lake Paul
Sent: Thursday, December 09, 2010 1:26 PM
To: 'Roy Hantgan'; rasmb at rasmb.bbri.org
Subject: Re: [RASMB] radial scans 6 sector cells



Roy,

Funny you should mention this, I did my first 6-sector experiment yesterday.
The 5.7 default might not cover the inside sector, so you might have to
adjust it to 5.65. I did this by trial and error and by getting help from
Dr. John Burgner. The 5.65 worked well I get alittle of the top of the cell
and both menisci. Hope this helps.

Lake

 

From: rasmb-bounces at rasmb.bbri.org [mailto:rasmb-bounces at rasmb.bbri.org] On
Behalf Of Roy Hantgan
Sent: Thursday, December 09, 2010 3:42 PM
To: rasmb at rasmb.bbri.org
Subject: [RASMB] radial scans 6 sector cells

 

Dear colleagues:

 

Can you please remind me how you set the radial distance for sed eq runs in
6-sector cells?
I've only run sed eq in double sector cells. 

 

Thanks for your advice,

Roy 

 

Roy  R. Hantgan, Ph.D.

Associate Professor of Biochemistry / Associate in Molecular Medicine

Director, Macromolecular Interactions Core Laboratory (MICL)

Wake Forest University School of Medicine

3058 Hanes Bldg / TEL 336-716-4675

 

 

 

-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://list.rasmb.org/pipermail/rasmb-rasmb.org/attachments/20101209/f84b2876/attachment.htm>