[RASMB] Multi-wavelength SE crash

[John Philo]

Mark, I have not had similar problems when using multiple wavelengths for
absorbance, so I am going to guess that this is a software bug related to
trying to save these scans as intensity rather than absorbance. You could
test that theory pretty quickly if you also drop the replicates and up the
radial spacing.
 
In general I would recommend filling the cells in a pattern that minimizes
the number of wavelength changes. That is, I typically will group samples at
similar concentrations together in the same cell so fewer changes are
needed.
 
John

  _____  

From: rasmb-bounces at rasmb.bbri.org [mailto:rasmb-bounces at rasmb.bbri.org] On
Behalf Of Mark Agacan
Sent: Thursday, September 16, 2010 7:33 AM
To: rasmb at server1.bbri.org
Subject: [RASMB] Multi-wavelength SE crash


Hello,
 
I'm trying to run a sedimentation equilibrium experiment at 3 wavelengths.
W1 is 280 nm , W2 is 250 nm and W3 is 230 nm.  The first scan completes ok
at W1, but at the second wavelength the software crashes just before it
completes the scan.  
 
I've heard that this could be due to the software version I'm using, v.4.5.
 
Other parameters are:
Step mode; 20 replicates; 0.001 radial step size; 6-channel centerpiece;
8-hole rotor; one cell in position 4, counterbalance in position 8;
Acquiring intensity instead of OD data; Absorbance scans only; 10 000 rpm;
 
Has anyone had similar problems and found a remedy?
 
Many Thanks,
 
Mark
 
 
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Dr Mark Agacan, Scientific Officer for the Division of Biological Chemistry
and Drug Discovery,
Wellcome Trust Biocentre, College of Life Sciences, University of Dundee,
Dundee, DD1 5EH 
Tel: +44 1382 386095    Fax: +44 1382 345764    Mobile: 07525 451 117
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 
 

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