[RASMB] Multi-wavelength SE crash

[Mark Agacan]

Hello,
 
I'm trying to run a sedimentation equilibrium experiment at 3 wavelengths.  W1 is 280 nm , W2 is 250 nm and W3 is 230 nm.  The first scan completes ok at W1, but at the second wavelength the software crashes just before it completes the scan.  
 
I've heard that this could be due to the software version I'm using, v.4.5.
 
Other parameters are:
Step mode; 20 replicates; 0.001 radial step size; 6-channel centerpiece; 8-hole rotor; one cell in position 4, counterbalance in position 8; Acquiring intensity instead of OD data; Absorbance scans only; 10 000 rpm;
 
Has anyone had similar problems and found a remedy?
 
Many Thanks,
 
Mark
 
 
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Dr Mark Agacan, Scientific Officer for the Division of Biological Chemistry and Drug Discovery,
Wellcome Trust Biocentre, College of Life Sciences, University of Dundee, Dundee, DD1 5EH 
Tel: +44 1382 386095    Fax: +44 1382 345764    Mobile: 07525 451 117
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