[RASMB] 99% versus >99% guanidine HCl

[Titus M. Franzmann]

Hi, 
>99% is typically also considered to be spectroscopically pure and is
recommended for spectroscopic measurements/assays such as protein
unfolding/refolding. It is typically less than 0.1 Abs at a concentration
6M. If you need it for e.g. inclusion body resolubilization, etc. the less
expensive one is just as good.
Best
Titus.

-----Original Message-----
From: rasmb-bounces at server1.bbri.org [mailto:rasmb-bounces at server1.bbri.org]
On Behalf Of Avi Chakrabartty
Sent: Monday, May 17, 2010 3:25 PM
To: rasmb at server1.bbri.org
Subject: [RASMB] 99% versus >99% guanidine HCl

Dear RASMB community,

Has anybody found a real difference between 99% guanidine HCl from Aldrich 
compared to >99% guanidine HCl from Sigma?

-------------------------------------------
Avi Chakrabartty, Ph.D., Professor 
Departments of Medical Biophysics and Biochemistry, 
University of Toronto, Ontario Cancer Institute, 
MaRS Centre, 101 College St., Toronto, Ontario, M5G 1L7, CANADA 
Phone: 416-581-7553 Lab: 416-581-7555 Fax: 416-581-7555
email: chakrab at uhnres.utoronto.ca
--------------------------------------------

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