[RASMB] determination of phage particle concentration

Steve Harding Steve.Harding at nottingham.ac.uk
Fri Mar 5 06:44:20 PST 2010


Dear Sabine
Simple turbidity measurements (making sure you are away from absorption maxima) using a good quality spectrophotometer may suffice for the sort of information you are after - particle concentration, although stricvtly speaking it is not a hydrodynamic method..  This is is the usual method for measuring concentrations (particles per ml) of very large assemblies such as spores and I think it works for smaller assemblies that are not too dilute..
Victor Bloomfield's group did quite a bit on the turbidity of phages in the late 70's and gave the relevant formulae (you may need the approx  mol. wt value) - if you look up Bahls and Bloomfield (and not Victor's QLS papers) that should give you a lead!
All best
Steve Harding
 
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> Dear all,
>
> we are trying to obtain the concentration of bacteriophages in a
> solution, concentration meaning the number of particles per ml. The
> solution is supposed to be monodisperse and the MW is (roughly) known
> but could be determined exactly. Is the any idea of how to do this
> with a hydrodynamic method (staining is difficult to compare between
> phage mutants and counting infected cells is tedious). I`ve got the
> "feeling" that one could use the number-averaged MW, but maybe this is
> totally wrong.
>
> Thank you for any hints, literature is also welcome.
>
> Cheers,
>
> Sabine
>
>
>
>
> Sabine Kaltofen
> PhD student
>
> Universität Potsdam
> Department of Physical Biochemistry
> Institute of Biochemistry and Biology
> Karl-Liebknecht-Str. 24-25, Haus 25, Raum B/0.05
> D-14476 Potsdam-Golm
> Telefon: +49-(331)-977-5245
> Email: kaltofen at uni-potsdam.de
>
>
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