[RASMB] determination of phage particle concentration

[Timothy Dafforn]

depending which phage it is you can get a good idea of concentration from the OD280 
for instance for M13 the major protein, by far, is pVIII. the sequence for this protein is available so you can calculate an extinction coefficient. If you then assume 2500 copies per phage particle then you can get a concentration.. (its a bit approximate).
If it is M13, I have a txt doc with details..
Cheers
tim
________________________________________
From: rasmb-bounces at rasmb.bbri.org [rasmb-bounces at rasmb.bbri.org] On Behalf Of kaltofen at uni-potsdam.de [kaltofen at uni-potsdam.de]
Sent: 04 March 2010 10:57
To: rasmb at server1.bbri.org
Cc: dandres at uni-potsdam.de
Subject: [RASMB] determination of phage particle concentration

Dear all,

we are trying to obtain the concentration of bacteriophages in a
solution, concentration meaning the number of particles per ml. The
solution is supposed to be monodisperse and the MW is (roughly) known
but could be determined exactly. Is the any idea of how to do this
with a hydrodynamic method (staining is difficult to compare between
phage mutants and counting infected cells is tedious). I`ve got the
"feeling" that one could use the number-averaged MW, but maybe this is
totally wrong.

Thank you for any hints, literature is also welcome.

Cheers,

Sabine




Sabine Kaltofen
PhD student

Universität Potsdam
Department of Physical Biochemistry
Institute of Biochemistry and Biology
Karl-Liebknecht-Str. 24-25, Haus 25, Raum B/0.05
D-14476 Potsdam-Golm
Telefon: +49-(331)-977-5245
Email: kaltofen at uni-potsdam.de



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