[RASMB] determination of phage particle concentration

[kaltofen at uni-potsdam.de]

Dear all,

we are trying to obtain the concentration of bacteriophages in a  
solution, concentration meaning the number of particles per ml. The  
solution is supposed to be monodisperse and the MW is (roughly) known  
but could be determined exactly. Is the any idea of how to do this  
with a hydrodynamic method (staining is difficult to compare between  
phage mutants and counting infected cells is tedious). I`ve got the  
"feeling" that one could use the number-averaged MW, but maybe this is  
totally wrong.

Thank you for any hints, literature is also welcome.

Cheers,

Sabine




Sabine Kaltofen
PhD student

Universität Potsdam
Department of Physical Biochemistry
Institute of Biochemistry and Biology
Karl-Liebknecht-Str. 24-25, Haus 25, Raum B/0.05
D-14476 Potsdam-Golm
Telefon: +49-(331)-977-5245
Email: kaltofen at uni-potsdam.de