[RASMB] density matching ASB-14

[Tina Daviter]

Dear All,

I will have to run an SV experiment in the presence of the detergent 
ASB-14 at 0.5%. It is a solid and I wondered if anyone has any 
information on the density of it in solution so I can do density 
matching? Or is it not that easy in this case?

The aim of the experiment is to get the oligomeric state of a membrane 
protein.

If the density is unavailable, any suggestion on how to most easily 
approach the problem would be highly welcome. I fear to determine the 
density by running ASB-14 in H20/D20 mixes might fail as it does not 
seem to absorb much at any wavelength (is interference sensitive enough?).

Also, I have not entirely understood how to account for the influence 
detergents have on the viscosity. I should be able to organise access to 
an Anton-Paar densitometer, but I don't think there is a viscosimeter 
that I could use.

Is SE inavoidable?

For more information on the detergent, if that helps, here is the full 
name and link to Sigma:
Amidosulfobetaine-14,3-[N,N-Dimethyl(3-myristoylaminopropyl)ammonio]propanesulfonate.
http://www.sigmaaldrich.com/catalog/ProductDetail.do?D7=0&N5=SEARCH_CONCAT_PNO|BRAND_KEY&N4=A1346|SIGMA&N25=0&QS=ON&F=SPEC

Literature recommendations (preferably overviews and experimental 
methods) are also welcome.

Thank you very much in advance.

Regards
Tina


-- 
Dr. Tina Daviter
ISMB Biophysics Centre
Birkbeck, Univ. of London
London, UK

T 020 70790749
E t.daviter at mail.cryst.bbk.ac.uk
W http://biophysics.ismb.lon.ac.uk