[RASMB] AUC Analysis for a protein without tryptophan

[Leech, AP]

Hi Rikita,

The reason for tryptophan is simply that it has a UV absorbance. If
there is no Trp but some Tyr or Phe, there may be enough absorbance.
Put your protein sequence into SEDNTERP and it will give you a handy
plot of absorbance vs wavelength so you can see if an alternative to
280 nm might be better. You can go to 230 nm or lower to measure
the peptide absorption but this is more liable to buffer interference.

Best regards,

Andrew

Ritika Sethi wrote:
> Hi
>  
> I am new to this technique.
> My protein precipitates in most of the buffers and one can't go beyond a 
> certain (very low) concentration. I was hoping to screen this protein 
> against different buffers and see micro-aggregates on the AUC and then 
> try different additives to remove the microaggregates. However, I 
> believe its impossible to do so if the protein is without tryptophan.
>  
> Any suggestions please?
>  
> Thanks
> 
> 
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-- 
Dr Andrew Leech                   *  Laboratory Head
Technology Facility               *  Molecular Interactions Laboratory
Department of Biology (Area 15)   *  Tel   : +44 (0)1904 328723
University of York                *  Fax   : +44 (0)1904 328804
PO Box 373,  York  YO10 5YW       *  Email : apl3 at york.ac.uk