[RASMB] AUC Analysis for a protein without tryptophan

Ritika Sethi Ritika.Sethi at sgc.ox.ac.uk
Thu Nov 26 04:34:05 PST 2009


Hi 
 
I am new to this technique. 
My protein precipitates in most of the buffers and one can't go beyond a certain (very low) concentration. I was hoping to screen this protein against different buffers and see micro-aggregates on the AUC and then try different additives to remove the microaggregates. However, I believe its impossible to do so if the protein is without tryptophan.
 
Any suggestions please?
 
Thanks
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