[RASMB] Hb tetramer

[Arthur Rowe]

Hi Mitra

An f/f0 value of 1.08 (±?) is low-ish, but within the ball park range for
'globular' proteins. I don't imagine that 2 mM Inositol would affect the
(properly and fully corrected) s value.

Regards

Arthur


Hello All
Two questions, related ones -

1. Is a S20,w value of 5.01 too high for a DEOXY Hb tetramer of 67180
Da MW? This gives a f/f0 of around 1.08. Just a reminder that deoxy Hb
tetramer does not dissociate to a dimer much (dissociation constant ~
10^-11 M).

2. can such an anomaly occur due to Hb interactions with the
components of the buffer - in this case 2 mM of Inositol
hexakisphosphate (IHP)?

Glad to hear any comments and/or references to papers.

Mitra
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