[RASMB] Fluorescence question

J. Preben Morth premo at bioxray.au.dk
Thu Jun 25 01:46:25 PDT 2009


> Hi
We are working on a soluble protein which show a constant increase in  
intrinsic Trp fluorescence changes
by plotting the 295nm/320nm ratio over time (up to 10min), we expect a  
drop. but the change seems to increase linearly? Can someone explain  
this, It might possibly be my lack of knowledge in this particular  
field.
The protein is soluble in water. and measured in water no ligands  
added or anything. When we add SDS the protein denatures and we see  
the expected downward bleaching curve.

cheers
Preben



Jens Preben Morth, Ph.D
Aarhus University
Department of Molecular Biology
Gustav Wieds Vej 10 C
DK - 8000 Aarhus C
Tel. +45 8942 5257, Fax. +45 8612 3178
jpm at mb.au.dk






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