[RASMB] FDS data fitting in Sedfit

[smcbryan]

I have recently collected SV data from clarified, whole-cell yeast extracts
which express GFP-tagged proteins of interest. Along with two of these
samples, I subjected the buffer and a non-GFP-expressing extract to SV. As
documented, there are 'autofluorescent' species in yeast extracts, in
particular a 4S (60 kDa) riboflavin binding protein which is complicating
my
analysis of the results. This species should show up in all three extract
samples, which is does not.
 I have the following question: does amplitude of the value on the Y-axis
from a c(s) fit
represent the fraction of each component (as seen on the X-axis), based
upon
its' contribution to the total signal from the sample, or does the peak
need to be integrated. 
thus, can I divide
each components c(s) into the total sedimenting fluorescent signal from the
sample to determine its' numerical fraction of the sample (ignoring
possible
quantum yield and quenching effects)?
thanks for any input. 
steve