[RASMB] upper concentration limit AUC

[Allen Minton]

Sedimentation equilibrium has been employed to study the properties 
of proteins at high concentrations for many years.  The following 
publications are just for starters:

R.C. Williams Jr. (1973) PNAS 70: 1506-1508.  A very short 
path-length cell was used to measure SE of hemoglobins A and S at 
concentrations up to over 250 g/L.

P.D. Ross et al. (1978) Biopolymers 17: 2285-8.  Another type of 
short path-length cell was used to measure SE of oxygenated and 
deoxygenated hemoglobins A and S at different temperatures at 
concentrations up to over 250 g/L.

A.P. Minton & M. Lewis (1981) Biophys. Chem. 14 317-324. A 
super-short path-length cell (ca .1 mm!) was used to measure SE of 
hemoglobin and myoglobin at concentrations up to over 350 g/L.

For interpretation of sedimentation equilibrium in highly nonideal 
protein solutions (WAY beyond second virial coefficient), see 
Zorrilla et al (2004).  Sedimentation equilibrium in a solution 
containing an arbitrary number of solute species at arbitrary 
concentrations: theory and application to concentrated solutions of 
ribonuclease. Biophys. Chem. 108 89-100.

Allen Minton


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