[RASMB] Sedfit peak integration

[Thomas Jowitt]

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I have a question regarding peak integration between different sedfit analyses. In an effort to gauge competition in a self-associating system, velocity was used to assess the level of dimerisation and subsequent competition using different concentrations of dimer and the competing element. The question is one of assessing the relative degree of competition, therefore the integration or relative size of the peaks. Is it right to normalize the different runs to the height of the dimer (predominant species), and therefore compare the relative size of the monomer peaks for competition, and if so can the integrated value of the normalized peaks be used also, or is that more subjective?

Thanks for any thoughts

Tom Jowitt
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