[RASMB] non-ideality in velocity [was: interference optics]

[Ewa Folta-Stogniew]

there is not much published that directly compares SEC-MALLS to SV, but in 
published study:

Gabrielson J P, Brader M. L., Pekar A. H., Mathis K. B., Winter G., 
Carpenter J. F., and Randolph T. W. (2007) Quantitation of aggregate levels 
in a recombinant humanized monoclonal antibody formulation by 
size-exclusion chromatography, asymmetrical flow field flow fractionation, 
and sedimentation velocity. J Pharm.Sci 96: 268-279


>Incidentally, it has been my experience that SEC-MALS is not so great
>at detecting large irreversible aggregates, because they can get stuck in
>the SEC column and you never see them in the MALS detector. However,
>the reversible oligomerization may be detected fine by MALS .

seems not be the case.

My guess would be that the reversible are harder for SEC-MALLS to account 
for due to a constant dilution on SEC and an introduction of another phase 
with which the protein can interact (SEC matrix) that may disturb the 
equilibrium.

Ewa
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