[RASMB] k_off rate

[Wenta Nikola]

Dear Colleagues!
Does anyone know how to calculate the k_off rate of a given dimer from it's kD? As the kD was found from Sedimentation Equilibrium, there are no kinetic information within. But there exists a crystal structure of that dimer, and with HydroPro, I obrained a diffusion coefficient for the dimer as well as for the monomer. Does anyone has any suggestions how to calculate my protein's k_off rate?
I am grateful for any suggestions.
Niko


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