[RASMB] parameters of beta-mercaptoethanol?
Borries Demeler
demeler at biochem.uthscsa.edu
Thu Mar 22 05:07:35 PDT 2007
>
> Hello all,
>
> TCEP is a good alternative reductant - again rather more expensive
> than DTT or BME, but much better optical properties than either.
>
> Andrew
I would like to second the use of TCEP. We have made excellent experiences
with TCEP - beats both BME and DTT in optical properties down to 260 nm.
I would also like to point out our CAUMA resource page to the community:
http://www.cauma.uthscsa.edu
click on "Resources" and check the left bar for many useful resources,
among them an absorbance comparison sheet for reductants, Xenon lamp profiles,
buffer pH/pka tables, buffer absorbance profiles and other goodies.
-Borries
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