[RASMB] parameters of beta-mercaptoethanol?

[Leech, AP]

Hello all,

TCEP is a good alternative reductant - again rather more expensive
than DTT or BME, but much better optical properties than either.

Andrew

Arthur Rowe wrote:
>     Dear Joy
> 
>     And - just one further point to add to Jo Butler's very correct
>     statements concerning BME - do make sure that any DTT you use is
>     newly supplied to you and made up immediately before use. Otherwise
>     the stuff oxidises, and is pretty much as bad as BME! Only much more
>     expensive . . . .
> 
>     Kind regards
> 
>     Arthur
> 
> --
> *******************************************************
> Arthur J Rowe
> Professor of Biomolecular Technology
> NCMH Business Centre
> University of Nottingham
> School of Biosciences
> Sutton Bonington
> Leicestershire LE12 5RD   UK
> 
> Tel:        +44 (0)115 951 6156
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> 
> 
> 
>     Dear Joy,
> 
>     Not directly answering your question, but just to point out that people
>     usually avoid beta-mercaptoethanol because of its UV absorption.  While
>     it may be fine with interference optics, it was no use with the older
>     absorbance, nor can one use D280 to get the initial protein
>     concentration.  this is why DTT is preferred - and this is in the
>     Sednterp parameters.
> 
>     Jo
> 
>     joylo at gate.sinica.edu.tw wrote:
>      > Dear all
>      >   I have the Sednterp (version 1.09) and it does not have any
>     information for beta-mercaptoethanol. I used two buffer systems. One
>     included 50mM Tris and 2mM b-ME at pH 7.5, and another was 50mM Tris
>     and 10mM b-ME at pH 7.5. Could anyone help me get the parameters
>     needed to include the b-ME used in my buffers?
>      > Thanks
>      > Joy Lo  
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> 
>     -- 
>     Dr P.J.G. Butler,
>     MRC Laboratory of Molecular Biology,
>     Hills Road, Cambridge, CB2 2QH, UK.
>     Tel. +44 (0)1223 402296
> 
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-- 
Dr Andrew Leech                   *  Laboratory Manager
Technology Facility               *  Molecular Interactions Laboratory
Department of Biology (Area 15)   *  Tel   : +44 (0)1904 328723
University of York                *  Fax   : +44 (0)1904 328804
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