[RASMB] V-bar of DHPC

[Jo Butler]

With respect to the dissociation with increasing detergent
concentration, you should look at the work of Karen Fleming (Johns
Hopkins), who has shown that this is equivalent to an increasing phase
volume and that dissociation is therefore to be expected.

Jo

columbia wrote:
>> Dear all,
>>
>> Does anybody have any idea about the partial specific volume of
>> dihexanoylphosphatidylcholine (DHPC)? And is there any difference in
>> V-bar when DHPC become micelle? Our proteins will be dissociated in
>> the presence of DHPC. The more DHPC, the more dissociation. I am
>> thinking the possibilities of our protein binding to DHPC micelle. I
>> appreciate if any comments.
>>
>>
>> Chi-Yuan Chou
>> Postdoctoral fellow,
>> Department of Biological Sciences, Columbia University, New York
>> 212-854-5221 
>
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-- 
Dr P.J.G. Butler,
MRC Laboratory of Molecular Biology,
Hills Road, Cambridge, CB2 2QH, UK.
Tel. +44 (0)1223 402296